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    As expected, m RNA levels of genes involved in arsenic methylation, including arsenic ( 3 oxidation state) methyltransferase (Cyt19), 5-methyltetrahydrofolate-homocysteine methyltransferase (Mtr), purine nucleoside phosphorylase (Pnp) and glutathione S-transferase omega 2 (Gstω2), were higher in the liver of 129 mice than B6 mice (Fig. m RNA levels of typical arsenic influx transporters, such as aquaglyceroporin (Aqp) 3, Aqp7 and Aqp9, showed a decreasing trend over time after arsenic challenge, but were not significantly different between B6 and 129 mice (Fig. Among six arsenic efflux transporters that we determined, m RNA levels were significantly lower for multidrug resistance-associated protein (Mrp) 1 and Mrp4 in the liver in 129 mice than B6 mice after arsenic challenge (Fig. According to cycle time of reverse transcription-quantitative polymerase chain reaction (RT-q PCR), m RNA levels for Mrp2 and Mrp3 were much more abundant than other Mrps in the liver (22 vs 28–30), indicating Mrp2 and Mrp3 may play a major role in arsenic efflux in the liver.


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